FGFR1 ELISA Kit

Catalog No. ABIN1569843

Product Details FGFR1 ELISA Kit

Target: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FGFR1 in rat tissue homogenates, cell lysates and other biological fluids.

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of this index.

Cross-Reactivity (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.115 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Material not included:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Sample Volume: 100 μL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Assay Procedure:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handling Advice: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Storage: 4 °C,-20 °C

Storage Comment: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Expiry Date: 12 months

Target Details for FGFR1

Target: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Alternative Name: FGFR1 (FGFR1 Products)

Synonyms: FGFR ELISA Kit, fgfr ELISA Kit, X1FGFR ELISA Kit, XFGFR-1 ELISA Kit, XFGFRA2 ELISA Kit, bfgfr ELISA Kit, cek ELISA Kit, fgfr1 ELISA Kit, flg ELISA Kit, flt-2 ELISA Kit, flt2 ELISA Kit, kal2 ELISA Kit, ogd ELISA Kit, xfgfr1 ELISA Kit, CG7223 ELISA Kit, CT22273 ELISA Kit, CT39172 ELISA Kit, DFGF-R1 ELISA Kit, DFGF-R2 ELISA Kit, DFR-1 ELISA Kit, DFR1 ELISA Kit, DFR1/DFGF-R2 ELISA Kit, DPR3 ELISA Kit, DTRK(FR1) ELISA Kit, Dfr-1 ELISA Kit, Dfr1 ELISA Kit, DmHD-38 ELISA Kit, Dmel\\CG7223 ELISA Kit, Dtk1 ELISA Kit, EMS2 ELISA Kit, FGF-R2 ELISA Kit, FGFR2 ELISA Kit, FR1 ELISA Kit, Fr1 ELISA Kit, HD-38 ELISA Kit, HTL/FGFR1 ELISA Kit, Htl ELISA Kit, Tk1 ELISA Kit, dtk1 ELISA Kit, i100 ELISA Kit, i150 ELISA Kit, i79 ELISA Kit, j372 ELISA Kit, cd331 ELISA Kit, fgfbr ELISA Kit, fgfr-1 ELISA Kit, hbgfr ELISA Kit, n-sam ELISA Kit, ach ELISA Kit, cd333 ELISA Kit, cek2 ELISA Kit, fgfr-3 ELISA Kit, hsfgfr3ex ELISA Kit, jtk4 ELISA Kit, BFGFR ELISA Kit, CD331 ELISA Kit, CEK ELISA Kit, FGFBR ELISA Kit, FGFR-1 ELISA Kit, FLG ELISA Kit, FLT-2 ELISA Kit, FLT2 ELISA Kit, HBGFR ELISA Kit, HH2 ELISA Kit, KAL2 ELISA Kit, N-SAM ELISA Kit, OGD ELISA Kit, bFGF-R-1 ELISA Kit, AW208770 ELISA Kit, Eask ELISA Kit, Fgfr-1 ELISA Kit, Flt-2 ELISA Kit, Hspy ELISA Kit, FLG-1 ELISA Kit, FGFRI ELISA Kit, x1fgfr ELISA Kit, cb231 ELISA Kit, sb:cb231 ELISA Kit, fibroblast growth factor receptor ELISA Kit, fibroblast growth factor receptor 1 S homeolog ELISA Kit, basic fibroblast growth factor receptor 1 ELISA Kit, heartless ELISA Kit, fibroblast growth factor receptor 1 ELISA Kit, fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) L homeolog ELISA Kit, Fibroblast growth factor receptor 1 ELISA Kit, fibroblast growth factor receptor 1 L homeolog ELISA Kit, fibroblast growth factor receptor 1a ELISA Kit, FGFR ELISA Kit, fgfr ELISA Kit, fgfr1.S ELISA Kit, CpipJ_CPIJ005849 ELISA Kit, htl ELISA Kit, fgfr1 ELISA Kit, fgfr3.L ELISA Kit, FGFR1 ELISA Kit, Fgfr1 ELISA Kit, fgfr1.L ELISA Kit, fgfr1a ELISA Kit

Background: Alternative name: CD331, FLT2, KAL2, CEK, FLG, BFGFR, N-SAM,Basic Fibroblast Growth Factor Receptor 1, Fms-Related Tyrosine Kinase-2, Pfeiffer Syndrome

Gene ID: 79114

UniProt: Q04589

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, S100 Proteins