(1 reference)-
- Target
- Calcein AM
- Detection Method
- Fluorometric
- Application
- Enzyme Activity Assay (EAA)
- Brand
- SensoLyte®
- Characteristics
- The SensoLyte® Calcein Cell Viability Assay Kit provides a convenient tool to detect live cells. This kit can be used in proliferation, cytotoxicity, invasion, adhesion, migration and other cell-based assays.
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- Comment
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Enzymatic Activity Assay kit
- Restrictions
- For Research Use only
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- Handling Advice
- Protect Component A from light and moisture.
- Storage
- -20 °C
- Storage Comment
- Store Component A at -20 °C. Component B can be stored at room temperature for convenience.
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: "Antiviral effect of strictinin on influenza virus replication." in: Antiviral research, Vol. 88, Issue 1, pp. 10-8, (2010) (PubMed).
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: "Antiviral effect of strictinin on influenza virus replication." in: Antiviral research, Vol. 88, Issue 1, pp. 10-8, (2010) (PubMed).
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- Target
- Calcein AM
- Background
- Calcein AM (calcein acetoxymethyl ester) is a non-fluorescent reagent that easily permeates cellular membrane. Once it permeates live cells, esterases remove the acetomethoxy group allowing calcein to bind to calcium, which results in a strong green fluorescence. The increase of fluorescence is proportional to the number of live cells. Inside dead cells, the non-fluorescent calcein AM remains intact due to the lack of active esterases. The fluorescent signal from the conversion of calcein AM to calcein can be monitored at Ex/Em=494nm /520 nm.
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