DHL™ Cell Viability and Proliferation Assay Kit

Catalog No. ABIN1882493

Product Details

Target: LDH

Detection Method: Fluorometric

Application: Enzyme Activity Assay (EAA)

Application Details

Comment:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Handling

Handling Advice: Keep away from light.

Storage: -20 °C

References

Karjalainen, Jaalouk, Bueso-Ramos, Zurita, Kuniyasu, Eckhardt, Marini, Lichtiger, OBrien, Kantarjian, Cortes, Koivunen, Arap, Pasqualini: "Targeting neuropilin-1 in human leukemia and lymphoma." in: Blood, Vol. 117, Issue 3, pp. 920-7, (2011) (PubMed).

Walsh, Damjanovski: "IGF-1 increases invasive potential of MCF 7 breast cancer cells and induces activation of latent TGF-?1 resulting in epithelial to mesenchymal transition." in: Cell communication and signaling : CCS, Vol. 9, Issue 1, pp. 10, (2011) (PubMed).

Roelants, Labar, de Meester, Havaux, Tabilio, Gambhir, Di Ianni, Bol, Bertrand, Vanoverschelde: "Comparison between adenoviral and retroviral vectors for the transduction of the thymidine kinase PET reporter gene in rat mesenchymal stem cells." in: Journal of nuclear medicine : official publication, Society of Nuclear Medicine, Vol. 49, Issue 11, pp. 1836-44, (2008) (PubMed).

Siegfried, Gubish, Rothstein, Queiroz de Oliveira, Stabile: "Signaling pathways involved in cyclooxygenase-2 induction by hepatocyte growth factor in non small-cell lung cancer." in: Molecular pharmacology, Vol. 72, Issue 3, pp. 769-79, (2007) (PubMed).

Target Details

Target: LDH

Background: Lactate dehydrogenase (LDH) exists ubiquitously in the cytoplasm of living mammalian cells and the measurement of cytoplasmic LDH activity is a well-accepted assay to quantify living cell numbers and indicate cell viablity. The DHL™ Cell Viability and Proliferation Assay Kit provides researchers with a convenient one-solution and one-step assay to count living cells in a culture and continuously monitor cell proliferation over time by measuring cytoplasmic LDH activity. In this kit, resazurin is used as a sensitive fluorogenic indicator. Resazurin is be converted to the strongly fluorescent resorufin (Ex/Em=560nm/590 nm) by cytoplasmic LDH. The kit can detect as few as 48 living cells with a linear range up to 5X104 cells (r2=0.99). This kit can also be used for high throughput screening of cell proliferation or cytotoxicity effect of a variety of compounds.