ESM1 ELISA Kit

Catalog No. ABIN1889318

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse ESM1. There is 1 publication available.

Quick Overview for ESM1 ELISA Kit (ABIN1889318)

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Binding Specificity: AA 20-184

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 23.4-1500 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details ESM1 ELISA Kit

Minimum Detection Limit: 23.4 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse ESM1/Endocan

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: W20-R184

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: W20-R184

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse ESM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ESM1 has been precoated onto 96-well plates. Standards(NSO, W20-R184) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ESM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ESM1 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/mL, 46.8pg/mL, 23.4pg/mL mouse ESM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse ESM1 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 56, Standard deviation: 1.85, CV(%): 3.3
• Sample 2: n=16, Mean(pg/ml): 673, Standard deviation: 31.6, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 1033, Standard deviation: 57, CV(%): 5.5,
• Sample 1: n=24, Mean(pg/ml): 47, Standard deviation: 1.93, CV(%): 4.1
• Sample 2: n=24, Mean(pg/ml): 660, Standard deviation: 34.98, CV(%): 5.3
• Sample 3: n=24, Mean(pg/ml): 1135, Standard deviation: 72.64, CV(%): 6.4

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for ESM1 ELISA Kit (ABIN1889318)

Chen, Shin, Eggold, Chung, Zhang, Lee, Sunwoo: "ESM1 mediates NGFR-induced invasion and metastasis in murine oral squamous cell carcinoma." in: Oncotarget, Vol. 7, Issue 43, pp. 70738-70749, (2018) (PubMed).

Target Details for ESM1

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Alternative Name: ESM1

Background:

Protein Function: Involved in angiogenesis, promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions (By similarity). .

Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM-1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2(fibroblast growth factor 2).

Synonyms: Endothelial cell-specific molecule 1,ESM-1,Esm1,

Full Gene Name: Endothelial cell-specific molecule 1

Cellular Localisation: Secreted.

Gene ID: 71690

UniProt: Q9QYY7

Pathways: Growth Factor Binding