NOTCH2NL ELISA Kit

Catalog No. ABIN2041984

Product Details NOTCH2NL ELISA Kit

Target: NOTCH2NL (Notch 2 N-terminal Like (NOTCH2NL))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.117 ng/mL

Application Details

Plate: Pre-coated

Protocol: Notch2 N-Terminal Like Protein (NOTCH2NL) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Notch2 N-Terminal Like Protein (NOTCH2NL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Notch2 N-Terminal Like Protein (NOTCH2NL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Notch2 N-Terminal Like Protein (NOTCH2NL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Notch2 N-Terminal Like Protein (NOTCH2NL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NOTCH2NL

Target: NOTCH2NL (Notch 2 N-terminal Like (NOTCH2NL))

Alternative Name: Notch2 N-Terminal Like Protein (NOTCH2NL) (NOTCH2NL Products)

Synonyms: N2N ELISA Kit, notch 2 N-terminal like ELISA Kit, NOTCH2NL ELISA Kit