CCL22 ELISA Kit

Catalog No. ABIN2042381

Product Details CCL22 ELISA Kit

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Brand: BioAssay™

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components: 96-well plate pre-coated with anti-CCL22/MDC, Human
Lyophilized recombinant CCL22/MDC, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human CCL22/MDC in cell culture supernates, serum and plasma (heparin, EDTA).Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares <35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CCL22

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Alternative Name: CCL22/MDC (CCL22 Products)

Synonyms: ABCD-1 ELISA Kit, DC/B-CK ELISA Kit, MDC ELISA Kit, SCYA22 ELISA Kit, STCP-1 ELISA Kit, DCBCK ELISA Kit, Scya22 ELISA Kit, Mdc ELISA Kit, CCL22 ELISA Kit, C-C motif chemokine ligand 22 ELISA Kit, chemokine (C-C motif) ligand 22 ELISA Kit, CCL22 ELISA Kit, Ccl22 ELISA Kit

Gene ID: 6367

UniProt: O00626