IL12 ELISA Kit
Quick Overview for IL12 ELISA Kit (ABIN2042521)
Target
See all IL12 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 7.8 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 2 pg/mL
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Components
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96-well plate pre-coated with anti-IL-12(p70), Human
Lyophilized recombinant IL-12(p70), Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Interleukin 12 (IL12) ELISA Kit
IL12 Reactivity: Rat Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Mouse Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Rabbit Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Chicken Colorimetric Competition ELISA 0.78 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Rat Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitSmall Sample IL12 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitHigh Sensitivity IL12 Reactivity: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Human IL-12(p70) in cell culture supernates and serum.Interleukin (IL)-12 is a 70kD cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response.[i] And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.[ii] Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.[iii] The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75kD.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- IL12 (Interleukin 12 (IL12))
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Alternative Name
- IL-12(p70)
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Gene ID
- 3592
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UniProt
- O60595
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Pathways
- JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
Target See all IL12 ELISA Kits
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