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IL-6 ELISA Kit

This Rat IL-6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat IL-6.
Catalog No. ABIN2042559

Quick Overview for IL-6 ELISA Kit (ABIN2042559)

Target

See all IL-6 (IL6) ELISA Kits
IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity

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  • 10
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  • 7
  • 4
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Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

62.5-4000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    62.5 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 5 pg/mL

    Components

    96-well plate pre-coated with anti-IL-6, Rat
    Lyophilized recombinant IL-6, Rat standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Rat IL-6 in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene.1 Interleukin-6 (IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion.2 IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption.3 IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone (CRH).4 Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor (IL-6Ralpha), and the shared signaling receptor gp130.5 The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kD.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all IL-6 (IL6) ELISA Kits

    IL-6 (IL6) (Interleukin 6 (IL6))

    Alternative Name

    IL-6

    Gene ID

    24498

    UniProt

    P20607

    Pathways

    TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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