TNF alpha ELISA Kit

Catalog No. ABIN2042688

This Rat TNF alpha ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat TNF alpha.

Quick Overview for TNF alpha ELISA Kit (ABIN2042688)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Product Details TNF alpha ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Characteristics: Specific detection range: 15.6pg/mL-1000pg/mL (Body fluids, tissue lysates or cell culture supernates), 7.8pg/mL-500pg/mL (Rat serum)

Components: 96-well plate pre-coated with anti-TNF alpha, Rat
Lyophilized recombinant TNF alpha, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Rat TNF alpha in cell culture supernates, serum and plasma (heparin, EDTA).Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer.1 Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology.2 TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro.3 TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids.4 TNF-alpha has been mapped to human chromosome 6.2 The standard product used in this kit is recombinant rat TNF , consisting of 157 amino acids with the molecular mass of 17kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Gene ID: 24835

UniProt: P16599

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome