alpha Fetoprotein ELISA Kit
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- Target See all alpha Fetoprotein (AFP) ELISA Kits
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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Reactivity
- Monkey
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.31-20 ng/mL
- Minimum Detection Limit
- 0.31 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.118 ng/mL
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- Discover our best selling AFP ELISA Kit
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- Discover our top product AFP ELISA Kit
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- Plate
- Pre-coated
- Protocol
- Alpha-Fetoprotein (aFP) BioAssay-ELISA Kit (Monkey) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fetoprotein (aFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fetoprotein (aFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fetoprotein (aFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Alpha-Fetoprotein (aFP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all alpha Fetoprotein (AFP) ELISA Kits
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
- Abstract
- AFP Products
- Synonyms
- FETA ELISA Kit, HPAFP ELISA Kit, alpha fetoprotein ELISA Kit, alpha-fetoprotein ELISA Kit, AFP ELISA Kit, Afp ELISA Kit
- Pathways
- C21-Steroid Hormone Metabolic Process
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