CHEK1 ELISA Kit

Catalog No. ABIN2043125

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse CHEK1.

Quick Overview for CHEK1 ELISA Kit (ABIN2043125)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details CHEK1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.058 ng/mL

Application Details

Plate: Pre-coated

Protocol: Checkpoint Homolog (CHEK1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Checkpoint Homolog (CHEK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Checkpoint Homolog (CHEK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Checkpoint Homolog (CHEK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Checkpoint Homolog (CHEK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: Checkpoint Homolog (CHEK1)

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair