C8B ELISA Kit

Catalog No. ABIN2043207

Product Details C8B ELISA Kit

Target: C8B (Complement Component 8, beta Polypeptide (C8B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.139 ng/mL

Application Details

Plate: Pre-coated

Protocol: Complement Component 8b (C8b) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 8b (C8b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 8b (C8b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 8b (C8b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Complement Component 8b (C8b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for C8B

Target: C8B (Complement Component 8, beta Polypeptide (C8B))

Alternative Name: Complement Component 8b (C8b) (C8B Products)

Synonyms: C82 ELISA Kit, 4930439B20Rik ELISA Kit, AI595927 ELISA Kit, complement C8 beta chain ELISA Kit, complement component 8, beta polypeptide ELISA Kit, C8B ELISA Kit, C8b ELISA Kit, c8b ELISA Kit

Pathways: Complement System