ATP5J ELISA Kit

Catalog No. ABIN2043233

Product Details ATP5J ELISA Kit

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.068 ng/mL

Application Details

Plate: Pre-coated

Protocol: Coupling Factor 6 (CF6) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coupling Factor 6 (CF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coupling Factor 6 (CF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coupling Factor 6 (CF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Coupling Factor 6 (CF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ATP5J

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Alternative Name: Coupling Factor 6 (CF6) (ATP5J Products)

Synonyms: zgc:77541 ELISA Kit, wu:fa66e04 ELISA Kit, ATP5 ELISA Kit, ATP5A ELISA Kit, ATPM ELISA Kit, CF6 ELISA Kit, F6 ELISA Kit, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 ELISA Kit, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 ELISA Kit, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 L homeolog ELISA Kit, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 ELISA Kit, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F ELISA Kit, atp5j ELISA Kit, ATP5J ELISA Kit, atp5j.L ELISA Kit, Atp5j ELISA Kit

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process