HNF1B ELISA Kit

Catalog No. ABIN2043635

Product Details HNF1B ELISA Kit

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.047 ng/mL

Application Details

Plate: Pre-coated

Protocol: Hepatocyte Nuclear Factor 1 Beta (HNF1b) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Nuclear Factor 1 Beta (HNF1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Hepatocyte Nuclear Factor 1 Beta (HNF1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Alternative Name: Hepatocyte Nuclear Factor 1 beta (HNF1b) (HNF1B Products)

Synonyms: FJHN ELISA Kit, HNF-1B ELISA Kit, HNF1beta ELISA Kit, HNF2 ELISA Kit, HPC11 ELISA Kit, LF-B3 ELISA Kit, LFB3 ELISA Kit, MODY5 ELISA Kit, TCF-2 ELISA Kit, TCF2 ELISA Kit, VHNF1 ELISA Kit, HNF-1b ELISA Kit, HNF1B ELISA Kit, AI385728 ELISA Kit, AI987804 ELISA Kit, HNF-1Beta ELISA Kit, Hnf1beta ELISA Kit, Tcf-2 ELISA Kit, Tcf2 ELISA Kit, vHNF1 ELISA Kit, vHNF-1 ELISA Kit, chunp6877 ELISA Kit, hnf1b ELISA Kit, mst ELISA Kit, tcf2 ELISA Kit, vhnf1 ELISA Kit, lfb3 ELISA Kit, hnf-1beta ELISA Kit, hnf1-beta ELISA Kit, hnf1beta ELISA Kit, HNF1g ELISA Kit, wu:fc23f06 ELISA Kit, HNF1 homeobox B ELISA Kit, HNF1 homeobox Ba ELISA Kit, HNF1 homeobox B S homeolog ELISA Kit, HNF1 homeobox B L homeolog ELISA Kit, HNF1 homeobox Bb ELISA Kit, HNF1B ELISA Kit, Hnf1b ELISA Kit, hnf1ba ELISA Kit, hnf1b.S ELISA Kit, hnf1b.L ELISA Kit, hnf1bb ELISA Kit

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation