IL-6 ELISA Kit

Catalog No. ABIN2043681

This Rat IL-6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat IL-6.

Quick Overview for IL-6 ELISA Kit (ABIN2043681)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 pg/mL

Application: ELISA

Product Details IL-6 ELISA Kit

Minimum Detection Limit: 1.56 pg/mL

Sensitivity: < 0.48 pg/mL

Application Details

Plate: Pre-coated

Protocol: High Sensitivity Interleukin 6 (IL6) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome