HTN1 ELISA Kit

Catalog No. ABIN2043697

Product Details HTN1 ELISA Kit

Target: HTN1 (Histatin 1 (HTN1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-100 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: 0.59 ng/mL

Components: A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A (green), 1x120 µL
E: Detection Reagent B (red), 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30X, 1x20 mL

Application Details

Plate: Pre-coated

Protocol: Histatin 1 (HTN1) BioAssay-ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of HTN1 in human saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histatin 1 (HTN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histatin 1 (HTN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histatin 1 (HTN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Histatin 1 (HTN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HTN1

Target: HTN1 (Histatin 1 (HTN1))

Abstract: HTN1 Products

Synonyms: HIS1 ELISA Kit, histatin 1 ELISA Kit, HTN1 ELISA Kit