PDX1 ELISA Kit (Pancreatic and Duodenal Homeobox 1)

Details for Product PDX1 ELISA Kit No. ABIN2043763, Supplier: Log in to see
Antigen
  • GSF
  • IDX-1
  • IPF1
  • IUF1
  • MODY4
  • PDX-1
  • STF-1
  • ipf1
  • PDX1
  • IPF-1
  • Ipf1
  • Mody4
  • pdx-1
  • Idx1
  • Stf1
  • zgc:100874
  • XlHbox-8
  • xlhbox8
  • pancreatic and duodenal homeobox 1
  • pancreatic and duodenal homeobox 1 S homeolog
  • PDX1
  • pdx1
  • Pdx1
  • pdx1.S
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
7
6
2
Method Type
Sandwich ELISA
Detection Range
0.15-10 ng/mL
Minimum Detection Limit
0.15 ng/mL
Application
ELISA
Options
Supplier
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Supplier Product No.
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Brand BioAssay™
Detection Method Colorimetric
Sensitivity < 0.053 ng/mL
Plasmids, Primers & others Plasmids, Primers & others PDX1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Insulin Promoter Factor 1 (IPF) (PDX1 ELISA Kit Abstract)
Pathways Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location
Plate Pre-coated
Protocol Insulin Promoter Factor 1 (IPF) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Promoter Factor 1 (IPF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Promoter Factor 1 (IPF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Promoter Factor 1 (IPF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Insulin Promoter Factor 1 (IPF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C