NFIB ELISA Kit

Catalog No. ABIN2044115

Product Details NFIB ELISA Kit

Target: NFIB (Nuclear Factor I/B (NFIB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: Nuclear Factor I/B (NFIB) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor I/B (NFIB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor I/B (NFIB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor I/B (NFIB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Nuclear Factor I/B (NFIB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NFIB

Target: NFIB (Nuclear Factor I/B (NFIB))

Abstract: NFIB Products

Synonyms: NFIB ELISA Kit, nf1-b1 ELISA Kit, CTF ELISA Kit, HMGIC/NFIB ELISA Kit, NF-I/B ELISA Kit, NF1-B ELISA Kit, NFI-B ELISA Kit, NFI-RED ELISA Kit, NFIB2 ELISA Kit, NFIB3 ELISA Kit, 6720429L07Rik ELISA Kit, E030026I10Rik ELISA Kit, nuclear factor I B ELISA Kit, nuclear factor 1 B-type ELISA Kit, nuclear factor I B L homeolog ELISA Kit, nuclear factor I/B ELISA Kit, NFIB ELISA Kit, LOC100221557 ELISA Kit, nfib.L ELISA Kit, Nfib ELISA Kit