PRCP ELISA Kit
Quick Overview for PRCP ELISA Kit (ABIN2044291)
Target
See all PRCP ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.054 ng/mL
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Prolylcarboxypeptidase (PRCP) ELISA Kit
PRCP Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Prolylcarboxypeptidase (PRCP) ELISA KitPRCP Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Prolylcarboxypeptidase (PRCP) ELISA KitPRCP Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Prolylcarboxypeptidase (PRCP) ELISA KitPRCP Reactivity: Mouse Colorimetric Sandwich ELISA 37.5 pg/mL - 2400 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Prolylcarboxypeptidase (PRCP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolylcarboxypeptidase (PRCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolylcarboxypeptidase (PRCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolylcarboxypeptidase (PRCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Prolylcarboxypeptidase (PRCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- PRCP (Prolylcarboxypeptidase (PRCP))
Target See all PRCP ELISA Kits
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