WNT5B ELISA Kit

Catalog No. ABIN2044837

Product Details WNT5B ELISA Kit

Target: WNT5B (Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.53 ng/mL

Application Details

Plate: Pre-coated

Protocol: Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 5B (WNT5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for WNT5B

Target: WNT5B (Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B))

Alternative Name: Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) (WNT5B Products)

Synonyms: wnt-5b ELISA Kit, xwnt5b ELISA Kit, WNT5B ELISA Kit, AW545702 ELISA Kit, Wnt-5b ELISA Kit, xwnt-5c ELISA Kit, CHUNP6928 ELISA Kit, id:ibd5111 ELISA Kit, ppt ELISA Kit, wnt-5 ELISA Kit, wnt5 ELISA Kit, wnt[b] ELISA Kit, wu:fk85g06 ELISA Kit, Wnt family member 5B ELISA Kit, wingless-type MMTV integration site family, member 5B ELISA Kit, Wnt family member 5B S homeolog ELISA Kit, wingless-type MMTV integration site family, member 5b ELISA Kit, wnt5b ELISA Kit, WNT5B ELISA Kit, Wnt5b ELISA Kit, wnt5b.S ELISA Kit

Pathways: WNT Signaling, Embryonic Body Morphogenesis, Positive Regulation of fat Cell Differentiation