WFS1 ELISA Kit

Catalog No. ABIN2044853

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat WFS1.

Quick Overview for WFS1 ELISA Kit (ABIN2044853)

Target: WFS1 (Wolfram Syndrome 1 (WFS1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details WFS1 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.118 ng/mL

Application Details

Plate: Pre-coated

Protocol: Wolfram Syndrome Protein 1 (WFS1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wolfram Syndrome Protein 1 (WFS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wolfram Syndrome Protein 1 (WFS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wolfram Syndrome Protein 1 (WFS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Wolfram Syndrome Protein 1 (WFS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for WFS1

Target: WFS1 (Wolfram Syndrome 1 (WFS1))

Alternative Name: Wolfram Syndrome Protein 1 (WFS1)

Pathways: Sensory Perception of Sound, Carbohydrate Homeostasis, ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome