KIT ELISA Kit

Catalog No. ABIN2648535

This Human KIT ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human KIT.

Quick Overview for KIT ELISA Kit (ABIN2648535)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details KIT ELISA Kit

Purpose: Human CD117 ELISA is to be used for the in-vitro quantitative determination of Stem Cell Factor receptor molecule (CD117) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant CD117.

Analytical Method: Quantitative

Sensitivity: 0.095 ng/ml

Characteristics: Human CD117 ELISA Assay kit is for Research Use Only.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 1 - 2 h

Plate: Pre-coated

Protocol: The CD117 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD117 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD117 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD117 antigen and a biotinylated monoclonal antibody specific for CD117 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD117 present in the samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: CD117 / C-KIT

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity