Anti Endothelin Receptor A IgM Antibody ELISA Kit
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- Target
- Anti Endothelin Receptor A IgM Antibody
- Reactivity
- Human
- Detection Method
- Colorimetric
- Application
- ELISA
- Sample Type
- Serum, Plasma
- Sensitivity
- Cut-off
- Characteristics
- Endothelins (ET) are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. It mediates the effects through G-Protein-coupled receptors, the Endothelin receptors. There are two key receptor types, ETA and ETB. ETA receptors are found in the smooth muscle tissue of blood vessels where they increase vasoconstriction by ET-1. Human Anti Endothelin Receptor A IgA Antibody is designed for the determination of antibodies (IgM) against the Endothelin receptor subtype A in serum and plasma.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 μL
- Assay Time
- 3.5 h
- Protocol
- Human Anti Endothelin Receptor A IgM Antibody ELISA Assay EIA is an antibody screening test. Endothelin- receptor A has been pre-coated onto a microtiter plate. During the first incubation the anti-Endothelin receptor A-IgM-Antibodies of the samples are immobilised on the plate. The autoantibodies are detected with a POD labelled anti-human IgM antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-Endothelin receptor A-IgM-antibody.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target
- Anti Endothelin Receptor A IgM Antibody
- Target Type
- Antibody
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