Thyroglobulin ELISA Kit

Catalog No. ABIN2648834

Product Details Thyroglobulin ELISA Kit

Target: Thyroglobulin (TG)

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum

Specificity: Ultrasensitive ELISA

Sensitivity: 0.03 ng/mL

Characteristics: Biochemically, Tg is to be understood as a rather complex family of molecules. It is micro heterogeneous with inter- and intra-individual variations (iodination degree, carbohydrate contents etc.). Dimers and several fragments also exist. Additional heterogeneity is due to malignant de-differentiation. Specifically and unspecifically interfering factors in individual sera cause further problems. Therefore, the Tg determination still represents a rather ambitious method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 25 μL

Plate: Pre-coated

Protocol: Tg of serum samples, controls and calibrators react with the solid phase bound anti-hTg antibody during the first incubation period of 2.5 hours at room temperature. After removal of the non-bound components by a washing step anti-hTg HRP conjugate interacts with the Tg molecules trapped by the immobilized anti-hTg antibody during the second incubation at room temperature over night (> 15 hours). The sandwich type com-plexes formed are separated from unbound material by a further washing step. HRP converts the colorless substrate solution of 3,3',5,5'-tetramethylbenzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 15 min, turning the solution from blue to yellow.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Thyroglobulin

Target: Thyroglobulin (TG)

Alternative Name: Tg (Thyroglobulin) (TG Products)

Synonyms: TG ELISA Kit, cb717 ELISA Kit, AITD3 ELISA Kit, TGN ELISA Kit, Tgn ELISA Kit, cog ELISA Kit, thyroglobulin ELISA Kit, TG ELISA Kit, tg ELISA Kit, LOC701526 ELISA Kit, LOC701756 ELISA Kit, LOC100434297 ELISA Kit, Tg ELISA Kit

Pathways: Thyroid Hormone Synthesis