IL12 ELISA Kit

Catalog No. ABIN2690561

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL12. There are 2 publications available.

Quick Overview for IL12 ELISA Kit (ABIN2690561)

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8 - 500 pg/mL

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Supernatant

Product Details IL12 ELISA Kit

Minimum Detection Limit: 7.8 pg/mL

Purpose: The human IL-12 (p70) ELISA Kit is for the in vitro quantitative determination of human IL-12 (p70) in serum, plasma, and cell culture supernatant.

Analytical Method: Quantitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD OptEIA™ Human IL-12 (p70) ELISA Kit

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 1. Store unopened kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).

References for IL12 ELISA Kit (ABIN2690561)

Ling, Gately, Gubler, Stern, Lin, Hollfelder, Su, Pan, Hakimi: "Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 116-27, (1995) (PubMed).

Schoenhaut, Chua, Wolitzky, Quinn, Dwyer, McComas, Familletti, Gately, Gubler: "Cloning and expression of murine IL-12." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3433-40, (1992) (PubMed).

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12

Background: Human Interleukin-12 (p70) (hIL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein composed of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. The BD OptEIA™ kit specifically measures only this biologically active p70.
IL-12 stimulates the growth of activated CD4+ and CD8+ T cells and NK cells. IL-12 also promotes the development of proinflammatory-Th1-like CD4+ cells and cytotoxic CD8+ T cells. Other effects include increasing the lytic activity of NK cells and antibody-dependent cell-mediated cytotoxic cells, synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, and inducing NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome