IL12 ELISA Kit

Catalog No. ABIN2690562

This Human IL12 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IL12. This ELISA Kit has been cited in 2+ publications.

Quick Overview for IL12 ELISA Kit (ABIN2690562)

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Supernatant

Product Details IL12 ELISA Kit

Purpose: The Human IL-12 (p40) ELISA Kit II is for the quantitative determination of human IL-12 (p40) in serum, plasma, and cell culture supernatant.

Analytical Method: Quantitative

Application Details

Application Notes: The ELISA Kit II format was developed for superior accuracy with serum and plasma specimens. The data that demonstrates this enhancement can be located in the Performance "Recovery" and "Linearity" sections.

Comment:

BD OptEIA™ Human IL-12 (p40) ELISA Kit II

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 1. Store unopened kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).

References for IL12 ELISA Kit (ABIN2690562)

Ling, Gately, Gubler, Stern, Lin, Hollfelder, Su, Pan, Hakimi: "Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 116-27, (1995) (PubMed).

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, (1992) (PubMed).

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12

Background: Interleukin 12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35)and 40 kD (p40) subunits. The IL-12 p40 monomer shares amino acid sequence homology with the IL-6 receptor. It has been reported that activated PBMCs producea manyfold excess of IL-12 p40 monomer over the bioactive p70 heterodimer.The IL-12 p40 monomer has been reported to inhibit binding of IL-12 p70 to the IL-12 receptor, but with 20x less effectiveness than the IL-12 p70 homodimer.

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome