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JAML ELISA Kit

AMICA1 Reactivity: Human Colorimetric Sandwich ELISA 0.41-100 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2702816
  • Target See all JAML (AMICA1) ELISA Kits
    JAML (AMICA1) (Adhesion Molecule, Interacts with CXADR Antigen 1 (AMICA1))
    Reactivity
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.41-100 ng/mL
    Minimum Detection Limit
    0.41 ng/mL
    Application
    ELISA
    Purpose
    Human AMICA ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human AMICA.
    Sensitivity
    0.41 ng/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all JAML (AMICA1) ELISA Kits
    JAML (AMICA1) (Adhesion Molecule, Interacts with CXADR Antigen 1 (AMICA1))
    Alternative Name
    AMICA (AMICA1 Products)
    Synonyms
    RGD1562572 ELISA Kit, AMICA ELISA Kit, CREA7-1 ELISA Kit, CREA7-4 ELISA Kit, Gm638 ELISA Kit, JAML ELISA Kit, Crea7 ELISA Kit, junction adhesion molecule like ELISA Kit, Jaml ELISA Kit, JAML ELISA Kit
    Background
    Gene Names: AMICA1 JAML UNQ722/PRO1387
    Protein names: Junctional adhesion molecule-like (Adhesion molecule interacting with CXADR antigen 1) (Dendritic cell-specific protein CREA7-1)
    Gene ID
    120425
    UniProt
    Q86YT9
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