BMP15 ELISA Kit

Catalog No. ABIN2702863

Product Details BMP15 ELISA Kit

Target: BMP15 (Bone Morphogenetic Protein 15 (BMP15))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.6-150 ng/mL

Minimum Detection Limit: 0.6 ng/mL

Application: ELISA

Purpose: Human BMP-15 (GDF-9B) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human BMP-15. It shows no cross-reactivity with human BMP-1, -2, -3, -4, -5, -6, -7, -8, or -10.

Sensitivity: 0.6 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for BMP15

Target: BMP15 (Bone Morphogenetic Protein 15 (BMP15))

Alternative Name: BMP-15 (BMP15 Products)

Synonyms: GDF9B ELISA Kit, ODG2 ELISA Kit, POF4 ELISA Kit, AU015375 ELISA Kit, AU018861 ELISA Kit, AU021453 ELISA Kit, Bmp-15 ELISA Kit, C86824 ELISA Kit, C87336 ELISA Kit, GDF-9B ELISA Kit, Gdf-9b ELISA Kit, fi35a05 ELISA Kit, wu:fi35a05 ELISA Kit, bmp-15 ELISA Kit, BMP15 ELISA Kit, BMP-15 ELISA Kit, bone morphogenetic protein 15 ELISA Kit, BMP15 ELISA Kit, Bmp15 ELISA Kit, bmp15 ELISA Kit

Background: Gene Names: BMP15 GDF9B
Protein names: Bone morphogenetic protein 15 (BMP-15) (Growth/differentiation factor 9B) (GDF-9B)

Gene ID: 9210

UniProt: O95972