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FAS ELISA Kit

FAS Reactivity: Rat Colorimetric Sandwich ELISA 40-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2702995
$562.10
Plus shipping costs $45.00
Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target See all FAS ELISA Kits
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Reactivity
    • 8
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    40-10000 pg/mL
    Minimum Detection Limit
    40 pg/mL
    Application
    ELISA
    Purpose
    Rat Fas (TNFRSF6/Apo-1) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Rat FAS.
    Sensitivity
    40 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling FAS ELISA Kit
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all FAS ELISA Kits
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Alternative Name
    Fas (FAS Products)
    Synonyms
    ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6, FASL, TNFSF6, APT1LG1, CD178, CD95-L, CD95L, Fas-L, Faslg, Tnfsf6, gld, AI196731, APO1, TNFR6, Tnfrsf6, lpr, Fas cell surface death receptor, Fas ligand, Fas ligand (TNF superfamily, member 6), tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta, Fas (TNF receptor superfamily member 6), FAS, FASLG, Fasl, YWHAZ, Fas, fas
    Background
    Gene Names: Fas Apt1 Tnfrsf6
    Protein names: Tumor necrosis factor receptor superfamily member 6 (Apo-1 antigen) (Apoptosis-mediating surface antigen FAS) (FASLG receptor) (CD antigen CD95)
    Gene ID
    246097
    UniProt
    Q63199
    Pathways
    p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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