FIL1d ELISA Kit

Catalog No. ABIN2703197

Product Details FIL1d ELISA Kit

Target: FIL1d (Interleukin 1 delta (FIL1d))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.07-50 ng/mL

Minimum Detection Limit: 0.07 ng/mL

Application: ELISA

Purpose: Human IL-36 Ra (IL-1 F5) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 RA (IL-1 F3), IL-18 (IL-1 F4), IL-36 alpha (IL-1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-38 (IL-1 F10), IL-33 (IL-1 F11)

Sensitivity: 0.07 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for FIL1d

Target: FIL1d (Interleukin 1 delta (FIL1d))

Alternative Name: IL-1F5 (FIL1d Products)

Synonyms: AI413231 ELISA Kit, Fil1delta ELISA Kit, IL36RN ELISA Kit, Il-1h3 ELISA Kit, Il1hy1 ELISA Kit, FIL1 ELISA Kit, FIL1(DELTA) ELISA Kit, FIL1D ELISA Kit, IL1F5 ELISA Kit, IL1HY1 ELISA Kit, IL1L1 ELISA Kit, IL1RP3 ELISA Kit, IL36RA ELISA Kit, PSORP ELISA Kit, Il1f5 ELISA Kit, interleukin 1 family, member 5 (delta) ELISA Kit, interleukin 36 receptor antagonist ELISA Kit, Il1f5 ELISA Kit, IL36RN ELISA Kit, Il36rn ELISA Kit

Background: Gene Names: IL36RN FIL1D IL1F5 IL1HY1 IL1L1 IL1RP3 UNQ1896/PRO4342
Protein names: Interleukin-36 receptor antagonist protein (IL-36Ra) (FIL1 delta) (IL-1-related protein 3) (IL-1RP3) (Interleukin-1 HY1) (IL-1HY1) (Interleukin-1 delta) (IL-1 delta) (Interleukin-1 family member 5) (IL-1F5) (Interleukin-1 receptor antagonist homolog 1) (IL-1ra homolog 1) (Interleukin-1-like protein 1) (IL-1L1)

Gene ID: 26525

UniProt: Q9UBH0