Thymidine Phosphorylase ELISA Kit

Catalog No. ABIN2703395

Product Details Thymidine Phosphorylase ELISA Kit

Target: Thymidine Phosphorylase (TYMP)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.4-100 ng/mL

Minimum Detection Limit: 0.4 ng/mL

Application: ELISA

Purpose: Human PD-ECGF (Thymidine Phosphorylase) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human PD-ECGF. Other species not determined.

Sensitivity: 0.4 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Thymidine Phosphorylase

Target: Thymidine Phosphorylase (TYMP)

Alternative Name: PD-ECGF (TYMP Products)

Synonyms: ECGF ELISA Kit, ECGF1 ELISA Kit, MEDPS1 ELISA Kit, MNGIE ELISA Kit, MTDPS1 ELISA Kit, PDECGF ELISA Kit, TP ELISA Kit, hPD-ECGF ELISA Kit, 2900072D10Rik ELISA Kit, Ecgf1 ELISA Kit, PD-ECGF ELISA Kit, Pdgfec ELISA Kit, typh ELISA Kit, thymidine phosphorylase ELISA Kit, thymidine phosphorylase DeoA ELISA Kit, AMP phosphorylase ELISA Kit, Thymidine phosphorylase ELISA Kit, TYMP ELISA Kit, Tymp ELISA Kit, deoA ELISA Kit, Dd586_0614 ELISA Kit, Kvar_4413 ELISA Kit, NMAG_RS19330 ELISA Kit, Snov_1187 ELISA Kit, Fbal_2856 ELISA Kit, Ftrac_2071 ELISA Kit, Intca_1216 ELISA Kit, ARCVE_RS10415 ELISA Kit, Sphch_4174 ELISA Kit, Sinme_3461 ELISA Kit, METOK_RS06355 ELISA Kit, Mesop_1831 ELISA Kit, typh ELISA Kit

Background: Gene Names: TYMP ECGF1
Protein names: Thymidine phosphorylase (TP) (EC 2.4.2.4) (Gliostatin) (Platelet-derived endothelial cell growth factor) (PD-ECGF) (TdRPase)

Gene ID: 1890

UniProt: P19971

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2