VEGFB ELISA Kit

Catalog No. ABIN2703565

The Human VEGFB ELISA Kit (ABIN2703565) is a Colorimetric ELISA Kit designed to quantify Human VEGFB. This ELISA Kit has been cited in 1 publication.

Quick Overview for VEGFB ELISA Kit (ABIN2703565)

Target: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.4 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details VEGFB ELISA Kit

Minimum Detection Limit: 0.4 ng/mL

Purpose: Human VEGF-B ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human VEGF-B. Other species not determined.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 2 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for VEGFB ELISA Kit (ABIN2703565)

Liu, Xu, Jiao, Ren, Li: "Vascular endothelial growth factor B coordinates metastasis of non-small cell lung cancer." in: Tumour biology, Vol. 36, Issue 3, pp. 2185-91, (2015) (PubMed).

Target Details for VEGFB

Target: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Alternative Name: VEGF-B

Background:

Gene Symbols: VEGFB, VRF

Protein Name (Synonyms): Vascular endothelial growth factor B (VEGF-B) (VEGF-related factor) (VRF)

Gene ID: 7423

UniProt: P49765

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals