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MAP2K3 ELISA Kit

MAP2K3 Reactivity: Human, Mouse, Rat pSer189 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN2748359
  • Target See all MAP2K3 ELISA Kits
    MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))
    Binding Specificity
    pSer189, total
    Reactivity
    Human, Mouse, Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human/Mouse/Rat Phospho-MKK3 (S189) and Total MKK3 ELISA Kit. This assay semi-quantitatively measures phophorylated MKK3 (Ser189) and Total MKK3 in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human, mouse, and rat MKK3 phosphorylated at site Serine-189 and Total MKK3.
    Characteristics
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product MAP2K3 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all MAP2K3 ELISA Kits
    MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))
    Alternative Name
    MKK3 (MAP2K3 Products)
    Synonyms
    MAPKK3 ELISA Kit, MEK3 ELISA Kit, MKK3 ELISA Kit, PRKMK3 ELISA Kit, SAPKK-2 ELISA Kit, SAPKK2 ELISA Kit, AW212142 ELISA Kit, Prkmk3 ELISA Kit, mMKK3b ELISA Kit, Mkk3 ELISA Kit, mitogen-activated protein kinase kinase 3 ELISA Kit, mitogen activated protein kinase kinase 3 ELISA Kit, MAP kinase activator XMEK3 ELISA Kit, MAP2K3 ELISA Kit, Map2k3 ELISA Kit, map2k3 ELISA Kit
    Background
    Mitogen-activated protein kinase kinase 3 (MKK3) phosphorylated at Serine-189 and Total MKK3
    Gene ID
    5606
    UniProt
    P46734
    Pathways
    MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2
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