Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CDKN1B ELISA Kit

CDKN1B Reactivity: Mouse Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN2748475
  • Target See all CDKN1B ELISA Kits
    CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
    Reactivity
    • 7
    • 4
    • 4
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Mouse p27/Kip1 (CDKN1B) ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Mouse p27/Kip1 (CDKN1B)
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling CDKN1B ELISA Kit
    Top Product
    Discover our top product CDKN1B ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all CDKN1B ELISA Kits
    CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
    Alternative Name
    p27/Kip1 (CDKN1B Products)
    Synonyms
    CDKN4 ELISA Kit, KIP1 ELISA Kit, MEN1B ELISA Kit, MEN4 ELISA Kit, P27KIP1 ELISA Kit, cdkn1b ELISA Kit, sb:cb611 ELISA Kit, wu:fb63g10 ELISA Kit, wu:fb64g10 ELISA Kit, wu:fe18e03 ELISA Kit, CDKN1B ELISA Kit, Kip1 ELISA Kit, p27 ELISA Kit, FAM14D ELISA Kit, ISG12 ELISA Kit, ISG12A ELISA Kit, P27 ELISA Kit, Cdki1b ELISA Kit, AA408329 ELISA Kit, AI843786 ELISA Kit, p27Kip1 ELISA Kit, cdkn1bl ELISA Kit, cyclin dependent kinase inhibitor 1B ELISA Kit, cyclin-dependent kinase inhibitor 1Bb ELISA Kit, interferon alpha inducible protein 27 ELISA Kit, cyclin-dependent kinase inhibitor 1B ELISA Kit, cyclin-dependent kinase inhibitor 1Ba ELISA Kit, CDKN1B ELISA Kit, cdkn1bb ELISA Kit, IFI27 ELISA Kit, Cdkn1b ELISA Kit, cdkn1ba ELISA Kit
    Background
    P27/Kip1 (CDKN1B)
    Gene ID
    821314
    UniProt
    Q43735
    Pathways
    Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
You are here:
Support