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MBL2 ELISA Kit

MBL2 Reactivity: Mouse AA 19-244 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN2859303
  • Target See all MBL2 ELISA Kits
    MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))
    Binding Specificity
    AA 19-244
    Reactivity
    • 4
    • 4
    • 3
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    312-20000 pg/mL
    Minimum Detection Limit
    312 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MBL2
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: E19-D244
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: E19-D244
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 1 C-type lectin domain.

    Plate
    Pre-coated
    Protocol
    mouse MBL2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MBL2 has been precoated onto 96-well plates. Standards(NSO, E19-D244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MBL2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MBL2 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse MBL2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MBL2 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 3.2, Standard deviation: 0.157, CV(%): 4.9
    • Sample 2: n=16, Mean(ng/ml): 8.4, Standard deviation: 0.378, CV(%): 4.5
    • Sample 3: n=16, Mean(ng/ml): 12.8, Standard deviation: 0.474, CV(%): 3.7,
    • Sample 1: n=24, Mean(ng/ml): 3.6, Standard deviation: 0.23, CV(%): 6.4
    • Sample 2: n=24, Mean(ng/ml): 8.9, Standard deviation: 0.516, CV(%): 5.8
    • Sample 3: n=24, Mean(ng/ml): 13.1, Standard deviation: 0.707, CV(%): 5.4
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all MBL2 ELISA Kits
    MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))
    Alternative Name
    MBL2 (MBL2 Products)
    Synonyms
    COLEC1 ELISA Kit, HSMBPC ELISA Kit, MBL ELISA Kit, MBL2D ELISA Kit, MBP ELISA Kit, MBP-C ELISA Kit, MBP1 ELISA Kit, MBPD ELISA Kit, pMBP-27 ELISA Kit, mbl2 ELISA Kit, MBL1 ELISA Kit, cMBl ELISA Kit, collectin ELISA Kit, Ab2-001 ELISA Kit, Ab2-011 ELISA Kit, L-MBP ELISA Kit, MBL-C ELISA Kit, COLEC2 ELISA Kit, mbl ELISA Kit, etID42583.2 ELISA Kit, fb68b07 ELISA Kit, hbl3 ELISA Kit, wu:fb68b07 ELISA Kit, zgc:109836 ELISA Kit, mannose binding lectin 2 ELISA Kit, Mannose-binding protein C ELISA Kit, mannose-binding lectin (protein C) 2, soluble ELISA Kit, mannose-binding lectin (protein C) 2 ELISA Kit, mannose-binding lectin family member 3, pseudogene ELISA Kit, MBL2 ELISA Kit, mbl2 ELISA Kit, Mbl2 ELISA Kit, MBL3P ELISA Kit
    Background

    Protein Function: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity). .

    Background: MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

    Synonyms: Mannose-binding protein C,MBP-C,Mannan-binding protein,RA-reactive factor P28A subunit,RARF/P28A,Mbl2,

    Full Gene Name: Mannose-binding protein C

    Cellular Localisation: Secreted.
    Gene ID
    17195
    UniProt
    P41317
    Pathways
    Complement System, Positive Regulation of Immune Effector Process
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