KNG1 FLISA Kit
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- Target See all KNG1 FLISA Kits
- KNG1 (Kininogen 1 (KNG1))
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Reactivity
- Human, Mouse, Rat
- Detection Method
- Fluorometric
- Method Type
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all KNG1 FLISA Kits
- KNG1 (Kininogen 1 (KNG1))
- Alternative Name
- Bradykinin (KNG1 Products)
- Synonyms
- BDK FLISA Kit, BK FLISA Kit, KNG FLISA Kit, Kng FLISA Kit, KNG2 FLISA Kit, fb64g01 FLISA Kit, wu:fb64g01 FLISA Kit, zgc:103569 FLISA Kit, kininogen FLISA Kit, bdk FLISA Kit, kng FLISA Kit, KNG1 FLISA Kit, IHRP FLISA Kit, KINKG FLISA Kit, KINKH FLISA Kit, Kng1 FLISA Kit, Kngk FLISA Kit, kininogen 1 FLISA Kit, kininogen 1 L homeolog FLISA Kit, inter-alpha-trypsin inhibitor heavy chain family member 4 FLISA Kit, kininogen 2-like 1 FLISA Kit, KNG1 FLISA Kit, Kng1 FLISA Kit, kng1 FLISA Kit, kng1.L FLISA Kit, ITIH4 FLISA Kit, Kng2l1 FLISA Kit
- Pathways
- ACE Inhibitor Pathway, Glycosaminoglycan Metabolic Process
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