Chromogranin A FLISA Kit
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- Target See all Chromogranin A (CHGA) FLISA Kits
- Chromogranin A (CHGA)
- Binding Specificity
- AA 342-355, Preproprotein
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Reactivity
- Human
- Detection Method
- Fluorometric
- Method Type
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
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Chromogranin A (CHGA) ELISA Kit
CHGA Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum, Urine
Chromogranin A (CHGA) ELISA KitCHGA Reactivity: Rat Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA Reactivity: Mouse Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA Reactivity: Rat Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Chromogranin A (CHGA) ELISA KitCHGA Reactivity: Human Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Serum, Urine
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all Chromogranin A (CHGA) FLISA Kits
- Chromogranin A (CHGA)
- Alternative Name
- Chromogranin A (CHGA Products)
- Synonyms
- CGA FLISA Kit, CG-ALPHA FLISA Kit, FSHA FLISA Kit, GPHA1 FLISA Kit, GPHa FLISA Kit, HCG FLISA Kit, LHA FLISA Kit, TSHA FLISA Kit, ChrA FLISA Kit, fj05f09 FLISA Kit, si:dkey-177p2.2 FLISA Kit, wu:fj05f09 FLISA Kit, zgc:101749 FLISA Kit, zgc:56075 FLISA Kit, CHGA FLISA Kit, cgA FLISA Kit, chga FLISA Kit, chromogranin A FLISA Kit, glycoprotein hormones, alpha polypeptide FLISA Kit, uncharacterized CHGA FLISA Kit, chromogranin A S homeolog FLISA Kit, CHGA FLISA Kit, CGA FLISA Kit, Chga FLISA Kit, chga FLISA Kit, chga.S FLISA Kit
- Pathways
- Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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