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GALP FLISA Kit

GALP Reactivity: Human Fluorometric Competition ELISA
Catalog No. ABIN2871296
  • Target See all GALP FLISA Kits
    GALP (Galanin-Like Peptide (GALP))
    Reactivity
    • 2
    • 2
    • 1
    • 1
    Human
    Detection Method
    Fluorometric
    Method Type
    Competition ELISA
    Application
    ELISA
    Analytical Method
    Quantitative
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C
  • Target See all GALP FLISA Kits
    GALP (Galanin-Like Peptide (GALP))
    Alternative Name
    Alarin (GALP Products)
    Synonyms
    GALP FLISA Kit, galanin like peptide FLISA Kit, galanin-like peptide FLISA Kit, GALP FLISA Kit, ci-galp FLISA Kit, Galp FLISA Kit
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