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C1QTNF4 ELISA Kit

C1QTNF4 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4881998
  • Target See all C1QTNF4 products
    C1QTNF4 (Complement C1q Tumor Necrosis Factor-Related Protein 4 (C1QTNF4))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human C1qTNF4 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all C1QTNF4 products
    C1QTNF4 (Complement C1q Tumor Necrosis Factor-Related Protein 4 (C1QTNF4))
    Alternative Name
    C1qTNF4 (C1QTNF4 Products)
    Synonyms
    c1qt4 ELISA Kit, CTRP4 ELISA Kit, ZACRP4 ELISA Kit, 0710001E10Rik ELISA Kit, 9430004J15Rik ELISA Kit, zgc:112213 ELISA Kit, C1q and TNF related 4 ELISA Kit, C1q and tumor necrosis factor related protein 4 ELISA Kit, C1q and TNF related 4 S homeolog ELISA Kit, c1qtnf4 ELISA Kit, C1QTNF4 ELISA Kit, C1qtnf4 ELISA Kit, c1qtnf4.S ELISA Kit
    Gene ID
    114900
    UniProt
    Q9BXJ3
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