GCK ELISA Kit

Catalog No. ABIN4883035

Product Details GCK ELISA Kit

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Glucokinase/GCK ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes .

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for GCK

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Alternative Name: Glucokinase (GCK Products)

Synonyms: FGQTL3 ELISA Kit, GK ELISA Kit, GLK ELISA Kit, HHF3 ELISA Kit, HK4 ELISA Kit, HKIV ELISA Kit, HXKP ELISA Kit, LGLK ELISA Kit, MODY2 ELISA Kit, Gk ELISA Kit, Gls006 ELISA Kit, GCK ELISA Kit, BA4487 ELISA Kit, glk ELISA Kit, hk4 ELISA Kit, hhf3 ELISA Kit, hkiv ELISA Kit, hxkp ELISA Kit, gck-A ELISA Kit, mody2 ELISA Kit, glucokinase ELISA Kit, GLUKA ELISA Kit, RNGK2 ELISA Kit, glucokinase ELISA Kit, glucokinase (hexokinase 4) L homeolog ELISA Kit, glucokinase (hexokinase 4) ELISA Kit, GCK ELISA Kit, Gck ELISA Kit, glcK ELISA Kit, Bphyt_1025 ELISA Kit, Halhy_6548 ELISA Kit, gk ELISA Kit, gck.L ELISA Kit, gck ELISA Kit

Gene ID: 2645

UniProt: P35557

Pathways: MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process