HNF4A ELISA Kit

Catalog No. ABIN4883203

Product Details HNF4A ELISA Kit

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human HNF-4 alpha/NR2A1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes .

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF-4 alpha (HNF4A Products)

Synonyms: HNF4A ELISA Kit, HNF4alpha ELISA Kit, CG9310 ELISA Kit, DmHNF4 ELISA Kit, Dmel\\CG9310 ELISA Kit, HNF-4 ELISA Kit, HNF-4(D) ELISA Kit, HNF4 ELISA Kit, Hnf-4 ELISA Kit, Hnf-4h ELISA Kit, NR2A4 ELISA Kit, dHNF-4 ELISA Kit, dHNF4 ELISA Kit, Hnf4 ELISA Kit, Hnf4alpha ELISA Kit, MODY1 ELISA Kit, Nr2a1 ELISA Kit, Tcf14 ELISA Kit, HNF4a7 ELISA Kit, HNF4a8 ELISA Kit, HNF4a9 ELISA Kit, MODY ELISA Kit, NR2A1 ELISA Kit, NR2A21 ELISA Kit, TCF ELISA Kit, TCF14 ELISA Kit, HNF-4alpha ELISA Kit, fb58h09 ELISA Kit, id:ibd1279 ELISA Kit, wu:fb58h09 ELISA Kit, hnf4 ELISA Kit, nr2a1 ELISA Kit, hepatocyte nuclear factor 4 alpha ELISA Kit, Hepatocyte nuclear factor 4 ELISA Kit, hepatic nuclear factor 4, alpha ELISA Kit, hepatocyte nuclear factor 4, alpha ELISA Kit, hepatocyte nuclear factor 4 alpha S homeolog ELISA Kit, HNF4A ELISA Kit, hnf4a ELISA Kit, Hnf4 ELISA Kit, Hnf4a ELISA Kit, hnf4a.S ELISA Kit

Gene ID: 3172

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process