Plexin A4 ELISA Kit

Catalog No. ABIN4884197

Product Details Plexin A4 ELISA Kit

Target: Plexin A4 (PLXNA4)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Plexin A4 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Plexin A4

Target: Plexin A4 (PLXNA4)

Alternative Name: Plexin A4 (PLXNA4 Products)

Synonyms: D204 ELISA Kit, wu:fd49b01 ELISA Kit, wu:fe15f03 ELISA Kit, PLXNA4A ELISA Kit, FAYV2820 ELISA Kit, PLEXA4 ELISA Kit, PLXNA4B ELISA Kit, PRO34003 ELISA Kit, 9330117B14 ELISA Kit, Plxa4 ELISA Kit, mKIAA1550 ELISA Kit, PLEX2 ELISA Kit, Plxna4 ELISA Kit, PLXNA4 ELISA Kit, plexin A4 ELISA Kit, plexin-A4 ELISA Kit, plxna4 ELISA Kit, PLXNA4 ELISA Kit, LOC100467706 ELISA Kit, Plxna4 ELISA Kit

Gene ID: 91584

UniProt: Q9HCM2

Pathways: Regulation of Cell Size