TNC ELISA Kit

Catalog No. ABIN4884835

Product Details TNC ELISA Kit

Target: TNC (Tenascin C (TNC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6.1-1500 pg/mL

Minimum Detection Limit: 6.1 pg/mL

Application: ELISA

Purpose: Human Tenascin C ELISA Kit for serum, plasma, and cell culture supernatant samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human Tenascin C. Other species not determined.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples1,000 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for TNC

Target: TNC (Tenascin C (TNC))

Alternative Name: Tenascin C (TNC Products)

Synonyms: tn ELISA Kit, 150-225 ELISA Kit, GMEM ELISA Kit, GP ELISA Kit, HXB ELISA Kit, JI ELISA Kit, TN ELISA Kit, TN-C ELISA Kit, AI528729 ELISA Kit, C130033P17Rik ELISA Kit, Hxb ELISA Kit, Ten ELISA Kit, cytotactin ELISA Kit, tenascin-C ELISA Kit, P230 ELISA Kit, tenc ELISA Kit, wu:fk04d02 ELISA Kit, tenascin C L homeolog ELISA Kit, tenascin C ELISA Kit, tnc.L ELISA Kit, TNC ELISA Kit, MCYG_00667 ELISA Kit, tnc ELISA Kit, Tnc ELISA Kit

Gene ID: 3371

UniProt: P24821

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development