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FAS ELISA Kit

Mouse FAS ELISA Kit Colorimetric assay for quantification of Mouse FAS.
Catalog No. ABIN4885518

Quick Overview for FAS ELISA Kit (ABIN4885518)

Target

See all FAS ELISA Kits
FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity

  • 7
  • 5
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Application

ELISA

Sample Type

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
  • Purpose

    Custom Mouse FAS ELISA Kit.

    Analytical Method

    Quantitative

    Specificity

    The antibody pair provided in this kit recognizes Mouse FAS.

    Characteristics

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Components

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Material not included

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Sample Volume

    100 μL

    Plate

    Pre-coated

    Protocol

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Restrictions

    For Research Use only
  • Storage

    -20 °C

    Storage Comment

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Expiry Date

    6 months
  • Target See all FAS ELISA Kits

    FAS (TNF Receptor Superfamily, Member 6 (FAS))

    Alternative Name

    Fas

    Gene ID

    14102

    UniProt

    P25446

    Pathways

    p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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