FAS ELISA Kit

Catalog No. ABIN4885518

Mouse FAS ELISA Kit, Colorimetric assay for quantification of Mouse FAS.

Quick Overview for FAS ELISA Kit (ABIN4885518)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 8.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details FAS ELISA Kit

Minimum Detection Limit: 8.2 pg/mL

Purpose: Mouse FAS/TNFRSF6/Apo-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine (CCL21), Activin A, ADAMTS-1 (METH1), Adiponectin (ACRP30), Angiopoietin-3 (ANGPTL1), ANGPTL3, Artemin, CCL28 (MEC), CD36 (SR-B3), Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Galectin-7, gp130, Granzyme B, Gremlin-1, IFN-gamma R1, IL-17 RB, IL-17B, IL-22, MIP-1 beta (CCL4), MMP-10, MMP-2, MMP-3, PDGF-AA, Persephin, sFRP-3, SLAM (CD150), Sonic Hedgehog N-Terminal (Shh-N), TCK-1 (CXCL7), TECK (CCL25), TGF beta 1, Trance (TNFSF11), TREML1 (TLT-1), TWEAK (TNFSF12), VEGF-B, VEGFR.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 2 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4^°C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4^°C for up to 6 months. For extended storage, it is recommended to store at -80^°C.

Expiry Date: 6 months

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: Fas

Background:

Gene Symbols: FAS, APT1, TNFRSF6

Protein Name (Synonyms): Tumor necrosis factor receptor superfamily member 6 (Apo-1 antigen) (Apoptosis-mediating surface antigen FAS) (FASLG receptor) (CD antigen CD95)

Gene ID: 14102

UniProt: P25446

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity