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ATF2 ELISA Kit

ATF2 Reactivity: Human, Mouse pThr69 Colorimetric Sandwich ELISA Cell Culture Lysate
Catalog No. ABIN4885978
  • Target See all ATF2 ELISA Kits
    ATF2 (Activating Transcription Factor 2 (ATF2))
    Binding Specificity
    pThr69, pThr71, total
    Reactivity
    • 12
    • 10
    • 9
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human and Mouse Phospho-ATF2 (Thr69/71) and Total ATF2 ELISA Kit. This assay semi-quantitatively measures phophorylated ATF2 (Thr69/71) and Total ATF2 in lysate samples.
    Sample Type
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    This ELISA kit recognizes Human and Mouse ATF2 phosphorylated at site Threonine-69/71 as well as total ATF2.
    Characteristics
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all ATF2 ELISA Kits
    ATF2 (Activating Transcription Factor 2 (ATF2))
    Alternative Name
    ATF2 (ATF2 Products)
    Synonyms
    CRE-BP1 ELISA Kit, CREB2 ELISA Kit, HB16 ELISA Kit, TREB7 ELISA Kit, CG12850 ELISA Kit, CG3749 ELISA Kit, dATF-2 ELISA Kit, DmelCG30420 ELISA Kit, CG30420 ELISA Kit, CHN1 ELISA Kit, Atf-2 ELISA Kit, CRE-BP ELISA Kit, Creb2 ELISA Kit, D130078H02Rik ELISA Kit, D18875 ELISA Kit, Tg(Gzma-Klra1)7Wum ELISA Kit, mXBP ELISA Kit, atf2-A ELISA Kit, atf2l ELISA Kit, activating transcription factor 2 ELISA Kit, Activating transcription factor-2 ELISA Kit, activating transcription factor 2 S homeolog ELISA Kit, ATF2 ELISA Kit, Atf-2 ELISA Kit, Atf2 ELISA Kit, atf2.S ELISA Kit, atf2 ELISA Kit
    Gene ID
    1386
    UniProt
    P15336, P16951
    Pathways
    MAPK Signaling, RTK Signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades
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