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CHEK2 ELISA Kit

CHEK2 Reactivity: Human pThr68 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN4886026
  • Target See all CHEK2 ELISA Kits
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    Binding Specificity
    pThr68
    Reactivity
    • 6
    • 4
    • 3
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phospho-Chk2 (T68) ELISA Kit. This assay semi-quantitatively measures phophorylated Chk2 (Thr68) in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Human CHK2 phosphorylated at site Threonine-68.
    Characteristics
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product CHEK2 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all CHEK2 ELISA Kits
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    Alternative Name
    CHK2 (CHEK2 Products)
    Synonyms
    CDS1 ELISA Kit, CHK2 ELISA Kit, HuCds1 ELISA Kit, LFS2 ELISA Kit, PP1425 ELISA Kit, RAD53 ELISA Kit, hCds1 ELISA Kit, fa66f08 ELISA Kit, wu:fa66f08 ELISA Kit, zgc:55865 ELISA Kit, Cds1 ELISA Kit, HUCDS1 ELISA Kit, Rad53 ELISA Kit, Chk2 ELISA Kit, cds1 ELISA Kit, chek2 ELISA Kit, chk2 ELISA Kit, hucds1 ELISA Kit, lfs2 ELISA Kit, pp1425 ELISA Kit, rad53 ELISA Kit, checkpoint kinase 2 ELISA Kit, serine/threonine-protein kinase chk2 ELISA Kit, checkpoint kinase 2 L homeolog ELISA Kit, CHEK2 ELISA Kit, chek2 ELISA Kit, Chek2 ELISA Kit, MCYG_07308 ELISA Kit, chek2.L ELISA Kit
    Gene ID
    11200
    UniProt
    O96017
    Pathways
    p53 Signaling, Apoptosis, Cell Division Cycle
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