Lp-PLA2 (PLA2G7/PAF-AH) ELISA Kit

Details for Product No. ABIN4889779, Supplier: Log in to see
Antigen
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
8.19-2.000 ng/mL
Minimum Detection Limit
8.19 ng/mL
Application
ELISA
Options
Supplier
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Purpose Human Lp-PLA2/PLA2G7/PAF-AH ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA antibody pair recognizes Human Lp-PLA2.
Sensitivity 8.1 ng/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Antigen
Gene ID 7941
UniProt Q13093
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation

Recommended Dilution for serum and plasma samples2 fold

Restrictions For Research Use only
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
Supplier Images
ELISA image for Lp-PLA2 (PLA2G7/PAF-AH) ELISA Kit (ABIN4889779) Standard Curve
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