STAT5A ELISA Kit (Signal Transducer and Activator of Transcription 5A)

Details for Product STAT5A ELISA Kit No. ABIN4889793, Supplier: Log in to see
Antigen
  • MGF
  • STAT5
  • AA959963
  • Stat5
  • STAT5A/MGF
  • STATA5
  • STAT5A
  • stat5
  • stat5b
  • signal transducer and activator of transcription 5A
  • signal transducer and activator of transcription 5a
  • STAT5A
  • Stat5a
  • stat5a
  • LOC100348518
  • LOC100716811
Epitope
pTyr694, total
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
22
15
14
4
3
3
3
2
1
1
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Human Phospho-STAT5A (Tyr694) and Total STAT5A ELISA Kit. This assay semi-quantitatively measures STAT5A phosphorylated at Tyrosine-694 as well as total STAT5A in cell lysate samples.
Sample Type Cell Culture Lysate
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity This ELISA kit recognizes Human STAT5A phosphorylated at site Tyrosine-694 as well as total STAT5A.
Characteristics
  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Plasmids, Primers & others Plasmids, Primers & others STAT5A products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name STAT5A (STAT5A ELISA Kit Abstract)
Gene ID 6776
UniProt P42229
Pathways JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months
Supplier Images
ELISA image for Signal Transducer and Activator of Transcription 5A (STAT5A) ELISA Kit (ABIN4889793) A431 cells were treated with recombinant human EGF at 37°C for 20 min. Cells were...
ELISA image for Signal Transducer and Activator of Transcription 5A (STAT5A) ELISA Kit (ABIN4889793) A431 cells were treated or untreated with EGF. Cell lysates were analyzed using this ...
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