STAT5A ELISA Kit

Catalog No. ABIN4889793

Product Details STAT5A ELISA Kit

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Binding Specificity: pTyr694, total

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Phospho-STAT5A (Tyr694) and Total STAT5A ELISA Kit. This assay semi-quantitatively measures STAT5A phosphorylated at Tyrosine-694 as well as total STAT5A in cell lysate samples.

Sample Type: Cell Culture Lysate

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes Human STAT5A phosphorylated at site Tyrosine-694 as well as total STAT5A.

Characteristics:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Alternative Name: STAT5A (STAT5A Products)

Synonyms: MGF ELISA Kit, STAT5 ELISA Kit, AA959963 ELISA Kit, Stat5 ELISA Kit, STAT5A/MGF ELISA Kit, STATA5 ELISA Kit, STAT5A ELISA Kit, stat5 ELISA Kit, stat5b ELISA Kit, signal transducer and activator of transcription 5A ELISA Kit, signal transducer and activator of transcription 5a ELISA Kit, STAT5A ELISA Kit, Stat5a ELISA Kit, stat5a ELISA Kit, LOC100348518 ELISA Kit, LOC100716811 ELISA Kit

Gene ID: 6776

UniProt: P42229

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation