TANK-Binding Kinase 1 (TBK1) ELISA Kit

Details for Product No. ABIN4889797, Supplier: Log in to see
Antigen
  • nak
  • t2k
  • TBK1
  • wu:fk70c05
  • zgc:136548
  • NAK
  • T2K
  • 1200008B05Rik
  • AI462036
  • AW048562
  • TANK binding kinase 1 L homeolog
  • TANK binding kinase 1
  • TANK-binding kinase 1
  • tbk1.L
  • TBK1
  • tbk1
  • Tbk1
Epitope
pSer172, total
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
10
9
2
Method Type
Sandwich ELISA
Application
ELISA
Options
Supplier
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Purpose Human, Mouse and Rat Phospho-TBK1 (Ser172) and Total TBK1 ELISA Kit. This assay semi-quantitatively measures TBK1 phosphorylated at Serine-172 as well as total TBK1 in cell lysate samples.
Sample Type Cell Culture Lysate
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity This ELISA kit recognizes Human, Mouse and Rat TBK1 phosphorylated at site Serine-172 as well as total TBK1.
Characteristics
  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Antigen
Alternative Name TBK1 (TBK1 ELISA Kit Abstract)
Gene ID 29110
UniProt Q9UHD2, Q9WUN2
Pathways TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months
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